Abstrak
Hiperurisemia merupakan tingginya kadar asam urat dalam darah dan dapat menimbulkan penyakit gout. Salah satu obat yang digunakan untuk mengatasi hiperurisemia adalah allopurinol. Penggunaan obat tersebut menimbulkan berbagai efek samping jika digunakan dalam jangka waktu yang lama. Tumbuhan jelatang diketahui mengandung flavonoid yang dapat menurunkan kadar asam urat dalam darah. Studi ini dilakukan untuk menguji aktivitas tanaman jelatang dan untuk mengetahui dosis efektifnya dalam menurunkan kadar asam urat dalam darah pada mencit. Pada penelitian ini, ekstrak dibuat dengan menggunakan metode maserasi menggunakan etanol 70%. Uji dilakukan pada mencit yang diinduksi kalium oksonat dan jus hati ayam agar menaikkan kadar asam uratnya. Kemudian hewan uji dibagi menjadi 5 kelompok yakni terdiri dari kelompok kontrol negatif (NaCMC 0,5%BB), kontrol positif (allopurinol 13mg/kgBB), kelompok dosis I (125mg/kgBB), dosis II (250mg/kgBB), dan dosis III (500mg/kgBB). Selanjutnya diukur penurunan kadar asam urat darah mencit pada tiap jam selama 5 jam. Kemudian data penurunan kadar asam urat diolah secara statistik menggunakan metode Anova dan Duncan. Berdasarkan hasil uji pada mencit, ekstrak jelatang memiliki aktivitas antihiperurisemia dengan dosis berturut-turut adalah 500mg/kg BB (89,91%), 250mg/kg BB (73,7%), dan 125 mg/kg BB (63,56%).
Referensi
- Abdullah, Angelina, Yumna, M., Arbianti, R., Utami, T. S., Hermansyah, H., & Ningsih, S. (2018). Flavonoid isolation and identification of mother-in-law’s tongue leaves (sansevieria trifasciata) and the inhibitory activities to xanthine oxidase enzyme. E3S Web of Conferences. https://doi.org/10.1051/e3sconf/20186703011
- Abu-Gharbieh, E., Shehab, N. G., Almasri, I. M., & Bustanji, Y. (2018). Antihyperuricemic and xanthine oxidase inhibitory activities of Tribulus arabicus and its isolated compound, ursolic acid: In vitro and in vivo investigation and docking simulations. PLoS ONE. https://doi.org/10.1371/journal.pone.0202572
- Besral, B., & Widiantini, W. (2015). Determinan Stres pada Pegawai Kementerian Kesehatan Republik Indonesia. Kesmas: National Public Health Journal. https://doi.org/10.21109/kesmas.v9i3.568
- Carvalho, A. R., Costa, G., Figueirinha, A., Liberal, J., Prior, J. A. V., Lopes, M. C., Cruz, M. T., & Batista, M. T. (2017). Urtica spp.: Phenolic composition, safety, antioxidant and anti-inflammatory activities. Food Research International. https://doi.org/10.1016/j.foodres.2017.06.008
- Corp, N., & Pendry, B. (2013). The role of Western herbal medicine in the treatment of gout. Journal of Herbal Medicine. https://doi.org/10.1016/j.hermed.2013.08.002
- Dar, S. A., Ganai, F. A., Yousuf, A. R., Balkhi, M. U. H., Bhat, T. M., & Sharma, P. (2013). Pharmacological and toxicological evaluation of Urtica dioica. Pharmaceutical Biology. https://doi.org/10.3109/13880209.2012.715172
- Farag, M. A., Weigend, M., Luebert, F., Brokamp, G., & Wessjohann, L. A. (2013). Phytochemical, phylogenetic, and anti-inflammatory evaluation of 43 Urtica accessions (stinging nettle) based on UPLC-Q-TOF-MS metabolomic profiles. Phytochemistry. https://doi.org/10.1016/j.phytochem.2013.09.016
- Gansser, D., & Spiteller, G. (1995). Aromatase inhibitors from Urtica dioica roots. Planta Medica. https://doi.org/10.1055/s-2006-958033
- Grace, L. B. (2012). Goodman and gillman. In Elsevier.
- Hou, C., Liu, D., Wang, M., Gong, C., Li, Y., Yang, L., Yao, M., Yuan, E., & Ren, J. (2019). Novel xanthine oxidase-based cell model using HK-2?cell for screening antihyperuricemic functional compounds. Free Radical Biology and Medicine. https://doi.org/10.1016/j.freeradbiomed.2019.04.007
- Ilyas, M., Daud, N. S., & Aqmarina, M. (2019). Aktivitas Antihiperurisemia Ekstrak Etanol Daun Galing (Cayratia trifolia L. Domin) Pada Mencit BALB/C. WARTA FARMASI. https://doi.org/10.46356/wfarmasi.v8i2.88
- Katzung, & Trevor’s. (2015). Pharmacology Examination & Board Review. In Mc Graw Hill Educarion. https://doi.org/10.1007/978-1-4614-1174-1_13
- Kratzer, J. T., Lanaspa, M. A., Murphy, M. N., Cicerchi, C., Graves, C. L., Tipton, P. A., Ortlund, E. A., Johnson, R. J., & Gaucher, E. A. (2014). Evolutionary history and metabolic insights of ancient mammalian uricases. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1320393111
- Maiuolo, J., Oppedisano, F., Gratteri, S., Muscoli, C., & Mollace, V. (2016). Regulation of uric acid metabolism and excretion. International Journal of Cardiology. https://doi.org/10.1016/j.ijcard.2015.08.109
- Modarresi-Chahardehi, A., Ibrahim, D., Sulaiman, S. F., & Mousavi, L. (2012). Screening antimicrobial activity of various extracts of Urtica Dioica. Revista de Biologia Tropical. https://doi.org/10.15517/rbt.v60i4.2074
- Obertreis, B., Giller, K., Teucher, T., Behnke, B., & Schmitz, H. (1996). [Anti-inflammatory effect of Urtica dioica folia extract in comparison to caffeic malic acid]. Arzneimittel-Forschung. https://scholar.google.com/scholar_lookup?title=Anti-inflammatory%20effect%20of%20Urtica%20dioica%20folia%20extract%20in%20comparison%20to%20caffeic%20malic%20acid
- Salih, N. A. (2015). Effect of nettle (Urtica dioica) extract on gentamicin induced nephrotoxicity in male rabbits. Asian Pacific Journal of Tropical Biomedicine. https://doi.org/10.1016/j.apjtb.2015.07.005
- Setty, A. R., & Sigal, L. H. (2005). Herbal medications commonly used in the practice of rheumatology: Mechanisms of action, efficacy, and side effects. Seminars in Arthritis and Rheumatism. https://doi.org/10.1016/j.semarthrit.2005.01.011
