Nilai Rujukan Darah Rutin Dewasa Sehat di Kota Yogyakarta

Zulfikar Husni Faruq; Siti Nuryani; Sistiyono Sistiyono; Ullya Rahmawati

doi:10.36990/hijp.v17i1.1471

Authors

Zulfikar Husni Faruq Poltekkes Kemenkes Yogyakarta, Indonesia
Siti Nuryani Poltekkes Kemenkes Yogyakarta, Indonesia
Sistiyono Sistiyono Poltekkes Kemenkes Yogyakarta, Indonesia
Ullya Rahmawati Poltekkes Kemenkes Yogyakarta, Indonesia

DOI:

https://doi.org/10.36990/hijp.v17i1.1471

Keywords:

Reference Interval, Routine Blood Count, Referece Interval, Adult Health, Hematology

Abstract

Ringkasan: Latar belakang: Nilai rujukan darah rutin yang berasal dari populasi rujukan alat dan reagen berbeda dengan karakteristik populasi lokal, sehingga berpotensi tidak sesuai untuk interpretasi hasil laboratorium di Indonesia. Tujuan: Penelitian ini bertujuan menentukan nilai rujukan darah rutin pada dewasa sehat di Kota Yogyakarta. Metode: Penelitian analitik cross sectional dilakukan pada 241 pendonor darah sehat (120 pria, 121 wanita, usia 17-70 tahun) di PMI Kota Yogyakarta. Sampel darah dianalisis menggunakan Hematology Analyzer Sysmex XP-100. Data diuji normalitas dan perbedaan gender, nilai rujukan ditetapkan menggunakan persentil 2,5-97,5%. Hasil: Nilai rujukan untuk pria dan wanita berturut-turut: Hb (12,1-16,6; 11,5-15,3)g/dl, WBC (4,6-12,6; 5,3-13,1)×10³sel/?l, RBC (4,15-6,66; 3,99-5,49)×10?sel/?l, HCT (38-49,2; 35-45,5)%, PLT (184-453; 232-560)×10³sel/?l. Parameter MCV, MCH, MCHC, dan MPV tidak menunjukkan perbedaan signifikan berdasarkan gender. Simpulan: Nilai rujukan lokal menunjukkan perbedaan dengan standar rujukan yang umum digunakan laboratorium. Saran: Verifikasi nilai rujukan perlu dilakukan sebelum implementasi di laboratorium untuk meningkatkan akurasi interpretasi hasil pemeriksaan.

References

Abdullah, Dana Ahmed, Gizing Anwar Mahmood, and Heshu Sulaiman Rahman. 2020. “Hematology Reference Intervals for Healthy Adults of the City of Sulaymaniyah, Iraq.” International Journal of General Medicine Volume 13:1249–54. doi: 10.2147/IJGM.S270800.

Bachman, E., T. G. Travison, S. Basaria, M. N. Davda, W. Guo, M. Li, J. Connor Westfall, H. Bae, V. Gordeuk, and S. Bhasin. 2014. “Testosterone Induces Erythrocytosis via Increased Erythropoietin and Suppressed Hepcidin: Evidence for a New Erythropoietin/Hemoglobin Set Point.” The Journals of Gerontology Series A: Biological Sciences and Medical Sciences 69(6):725–35. doi: 10.1093/gerona/glt154.

Barale, Cristina, Elena Melchionda, Giulia Tempesta, Alessandro Morotti, and Isabella Russo. 2023. “Impact of Physical Exercise on Platelets: Focus on Its Effects in Metabolic Chronic Diseases.” Antioxidants 12(8):1609. doi: 10.3390/antiox12081609.

Bimerew, Lealem Gedefaw, Tesfaye Demie, Kaleab Eskinder, Aklilu Getachew, Shiferaw Bekele, Waqtola Cheneke, Zewdineh Sahlemariam, Wondimagegn Adisu, Yaregal Asres, Tilahun Yemane, Girum Tesfaye, Getnet Tesfaw, Esayas Kebede Gudina, and Zeleke Mekonnen. 2018. “Reference Intervals for Hematology Test Parameters from Apparently Healthy Individuals in Southwest Ethiopia.” SAGE Open Medicine 6:205031211880762. doi: 10.1177/2050312118807626.

Butkiewicz, Anna M., Halina Kemona, Violetta Dymicka-Piekarska, Joanna Matowicka-Karna, Piotr Radziwon, and Alina Lipska. 2006. “Platelet Count, Mean Platelet Volume and Thrombocytopoietic Indices in Healthy Women and Men.” Thrombosis Research 118(2):199–204. doi: 10.1016/j.thromres.2005.06.021.

Ceriotti, Ferruccio, Rolf Hinzmann, and Mauro Panteghini. 2009. “Reference Intervals: The Way Forward.” Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 46(1):8–17. doi: 10.1258/acb.2008.008170.

Esa, T., S. Aprianti, M. Arif, and Hardjoeno Hardjoeno. 2018. “NILAI RUJUKAN HEMATOLOGI PADA ORANG DEWASA SEHAT BERDASARKAN SYSMEX XT-1800i.” INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY 12(3):137–40. doi: 10.24293/ijcpml.v12i3.875.

Giles, C. 2008. “The Platelet Count and Mean Platelet Volume.” British Journal of Haematology 48(1):31–37. doi: 10.1111/j.1365-2141.1981.00031.x.

Henny, Joseph, Anne Vassault, Guilaine Boursier, Ines Vukasovic, Pika Mesko Brguljan, Maria Lohmander, Irina Ghita, Francisco A. Bernabeu Andreu, Christos Kroupis, Ludek Sprongl, Marc H. M. Thelen, Florent J. L. A. Vanstapel, Tatjana Vodnik, Willem Huisman, and Michel Vaubourdolle. 2016. “Recommendation for the Review of Biological Reference Intervals in Medical Laboratories.” Clinical Chemistry and Laboratory Medicine (CCLM) 54(12):1893–1900. doi: 10.1515/cclm-2016-0793.

Keohane, Elaine M., Jeanine M. Walenga, and Catherine N. Otto. 2016. Rodak’s Hematology Clinical Principles and Applications. 4th ed. St. Louis, Missouri: Elsevier Saunders.

Ma, Sijia, Juntong Yu, Xiaosong Qin, and Jianhua Liu. 2023. “Current Status and Challenges in Establishing Reference Intervals Based on Real-World Data.” Taylor & Francis Group 60(6):427–41. doi: https://doi.org/10.1080/10408363.2023.2195496.

Mengistu Sissay, Teklay, Melatwork Tibebu, Tagesachew Wasihun, and Aster Tsegaye. 2021. “Hematological Reference Intervals for Adult Population of Dire Dawa Town, East Ethiopia” edited by F. T. Spradley. PLOS ONE 16(2):e0244314. doi: 10.1371/journal.pone.0244314.

Murphy, William G. 2014. “The Sex Difference in Haemoglobin Levels in Adults — Mechanisms, Causes, and Consequences.” Blood Reviews 28(2):41–47. doi: 10.1016/j.blre.2013.12.003.

Nah, Eun-Hee, Suyoung Kim, Seon Cho, and Han-Ik Cho. 2018. “Complete Blood Count Reference Intervals and Patterns of Changes Across Pediatric, Adult, and Geriatric Ages in Korea.” Annals of Laboratory Medicine 38(6):503–11. doi: 10.3343/alm.2018.38.6.503.

Ozarda, Yesim. 2020. “Establishing and Using Reference Intervals.” Turkish Journal of Biochemistry 45(1):1–10. doi: 10.1515/tjb-2017-0299.

Özçürümez, Mustafa K., and Rainer Haeckel. 2018. “Biological Variables Influencing the Estimation of Reference Limits.” Scandinavian Journal of Clinical and Laboratory Investigation 78(5):337–45. doi: 10.1080/00365513.2018.1471617.

Pekelharing, J. M., O. Hauss, R. de Jonge, J. Lokhoff, J. Sodikromo, M. Spaans, R. Brouwer, S. de Lathouder, and R. Hinzmann. 2010. “Haematology Reference Intervals for Established and Novel Parameters in Healthy Adults.” 1.

Qiao, Rui, Shuo Yang, Bei Yao, Hongya Wang, Jie Zhang, and Hong Shang. 2014. “Complete Blood Count Reference Intervals and Age- and Sex-Related Trends of North China Han Population.” Clinical Chemistry and Laboratory Medicine (CCLM) 52(7):1025–32. doi: doi:10.1515/cclm-2012-0486.

Roshan, T. M., H. Rosline, S. A. Ahmed, M. Rapiaah, A. Wan Zaidah, and M. N. Khattak. 2009. “Hematological Reference Values of Healthy Malaysian Population.” International Journal of Laboratory Hematology 31(5):505–12. doi: 10.1111/j.1751-553X.2008.01068.x.

Samaneka, Wadzanai P., Gibson Mandozana, Willard Tinago, Nehemiah Nhando, Nyaradzo M. Mgodi, Mutsawashe F. Bwakura-Dangarembizi, Marshall W. Munjoma, Zvenyika A. R. Gomo, Zvavahera M. Chirenje, and James G. Hakim. 2016. “Adult Hematology and Clinical Chemistry Laboratory Reference Ranges in a Zimbabwean Population” edited by C. Johnson. PLOS ONE 11(11):e0165821. doi: 10.1371/journal.pone.0165821.

Santimone, I., A. Di Castelnuovo, A. De Curtis, M. Spinelli, D. Cugino, F. Gianfagna, F. Zito, M. B. Donati, C. Cerletti, G. De Gaetano, L. Iacoviello, and on behalf of the MOLI-SANI Project Investigators. 2011. “White Blood Cell Count, Sex and Age Are Major Determinants of Heterogeneity of Platelet Indices in an Adult General Population: Results from the MOLI-SANI Project.” Haematologica 96(8):1180–88. doi: 10.3324/haematol.2011.043042.

Suryaatmadja, Suryaatmadja. 2004. Tabel Konversi Sistem Satuan SI - Konvensional Dan Nilai Rujukan Dewasa - Anak Parameter Laboratorium Klinik. Perhimpunan Dokter Spesialis Patologi Klinik Indonesia Cabang Jakarta.

Tekke?in, Nilgun, Huseyin Bekoz, and Faruk Tukenmez. 2014. “The Largest Reference Range Study for Hematological Parameters from Turkey: A Case Control Study.” Journal of Clinical and Experimental Investigations 5(4). doi: 10.5799/ahinjs.01.2014.04.0455.

Wongkrajang, P., W. Chinswangwatanakul, C. Mokkhamakkun, N. Chuangsuwanich, B. Wesarachkitti, B. Thaowto, S. Laiwejpithaya, and O. Komkhum. 2018. “Establishment of New Complete Blood Count Reference Values for Healthy Thai Adults.” International Journal of Laboratory Hematology 40(4):478–83. doi: 10.1111/ijlh.12843.

Wu, Xinzhong, Min Zhao, Baishen Pan, Jie Zhang, Mingting Peng, Lanlan Wang, Xiaoke Hao, Xianzhang Huang, Runqing Mu, Wei Guo, Rui Qiao, Wenxiang Chen, Hong Jiang, Yueyun Ma, and Hong Shang. 2015. “Complete Blood Count Reference Intervals for Healthy Han Chinese Adults” edited by R. L. Schmidt. PLOS ONE 10(3):e0119669. doi: 10.1371/journal.pone.0119669.