Perbandingan Hasil Mikroskopis Basil Tahan Asam antara Pendiaman Carbol Fuchsin 5 dan 10 Menit Berdasarkan Skala IUATLD
DOI:
https://doi.org/10.36990/hijp.v18i3.1972Keywords:
Acid-Fast Bacilli, Carbol Fuchsin, IUATLD Scale, Tuberculosis, Acid-Fast Bacilli, Carbol Fuchsin, IUATLD Scale, Tuberculosis, Ziehl–NeelsenAbstract
Latar belakang: Rekomendasi durasi pendiaman Carbol Fuchsin pada pewarnaan Ziehl–Neelsen berbeda, yakni 5 menit pada petunjuk reagen dan minimal 10 menit pada pedoman nasional, sedangkan bukti mengenai kategori pembacaan IUATLD masih terbatas. Tujuan: Membandingkan hasil pembacaan mikroskopis BTA berdasarkan skala IUATLD antara pendiaman Carbol Fuchsin 5 dan 10 menit. Metode: Eksperimen laboratorium rancangan paired post-test only menggunakan 16 spesimen sputum positif BTA, masing-masing dibuat dua sediaan. Pembacaan dilakukan tersamar oleh dua pemeriksa. Analisis memakai uji Wilcoxon signed-rank eksak dan kappa berbobot linear. Hasil: Sebanyak 13 spesimen (81,25%) berkategori sama, sedangkan 3 spesimen (18,75%) meningkat dari 1+ menjadi 2+. Perbedaan tidak bermakna (p = 0,250). Kappa berbobot linear 0,829 (IK 95% 0,57–1,00). Simpulan: Belum terdapat bukti perbedaan kategori pembacaan, tetapi kesetaraan kedua durasi belum terbukti. Saran: Diperlukan penelitian multilaboratorium dengan spesimen lebih banyak, mencakup kategori negatif dan scanty; durasi 5 menit belum direkomendasikan menggantikan 10 menit
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