Neuroprotector Effects of Moringa Leaf (Moringa Oleifera Lamk) Ethanol Extract on Type Two Diabetes Rats Induced by Streptozotocin-Nicotinamid

Abstract

Moringa leaf (Moringa Oleifera Lamk) has been widely used as an alternative therapy for diabetes mellitus, supported by scientific evidence. However, the effect of moringa leaves on diabetic neuropathy has not been studied. This study aims to determine the timing of the occurrence of diabetic neuropathy and the neuroprotective activity of moringa leaf extract on streptozotocin-nicotinamide (STZ-NA) induction. STZ-NA was induced intra peritoneal for three days to obtain hyperglycemic conditions in rats, while reducing the amount of foods in test animals was carried out to get the nutritional deficiencies that will accelerate the occurrence of diabetic neuropathy. Intra peritoneal injection of 65 mg/kg bb STZ and 150 mg/kg bb NA were performed and observed until diabetic neuropathy occurred. Extract ethanol of Moringa leaf was given orally after the test animals had diabetic neuropathy on day 28 with doses of 50,100 and 150 mg/kg bb. Glibenclamide, Vitamin B6, Glibenclamide + vitamin B6 were used as medicines control. As result, lack of nutrition can accelerate the occurrence of diabetic neuropathy. Moringa leaf ethanol extract dose of 100, 150 mg/kg bb gave better activity than glibenclamide, but it’s not better than glibenclamide+vitamin B6. Diabetic neuropathy in malnourished rats occurred on day 28 after STZ-NA induction. The most potent dose of ethanol extract of moringa leaves to be developed as a neuroprotector is a dose of 150 mg/kg bb.