Early Identification Of The N-Acetyltransferase 2 (Nat2) Gene Polymorphism In The Batak

Abstract

Indonesia has various ethnic groups spread from Sabang to Merauke consisting of more than 300 ethnic groups, Research on the TB gene in Javanese (40%) Sundanese (15.50%), Malay (3.7%), Batak (3.6%), Madura (3%) and Betawi (2.88%). The aim is to determine the presence of polymorphisms in the NAT2 gene of healthy Batak subjects along with the types of polymorphisms and types of acetylators. The impact of NAT2 polymorphisms are drug toxicity, peripheral neuropathy.

The method uses the RFLP method with a sample of 10 Batak tribes. Blood samples were isolated by DNA using the Wizard® Genomic DNA Purification Kit then RFLP PCR using restriction enzymes: Taq1, Kpn1, BamH1 and electrophoresis.

The RFLP of the Kpn1 restriction enzyme used a 100bp marker. Taq1 obtained NAT2*4 and the mutant allele NAT2*6A. BamH1 obtained NAT2*4 and the mutant allele NAT2*7B. There are NAT2 gene polymorphisms in the Batak tribe, namely NAT2*4/*5B, NAT2*4/*6A, NAT2*4/*7B, NAT2*5B/*5B and NAT2*7B/*7B and the type of NAT2 gene polymorphism in the Batak tribe. namely medium acetylator NAT2*4/*5B (20%), NAT2*4/*6A (33.3%) and in NAT2*4/*7B (20%), slow acetylator NAT2*5B/*5B (13,3%) and NAT2*7B (13.3%). The types of acetylators in the Batak tribe of North Sumatra province are medium and slow acetylators.