Deteksi Metilasi Gen IGF2/H19 Pada DNA Sel Darah Tepi Anak Stunting Usia 3-5 tahun: Metilasi IGF2 pada anak stunting

Ahmad Utomo; razari; harliansyah harliansyah; syukrini bahri

doi:10.36990/hijp.v16i1.1123

Authors

Ahmad Utomo Universitas YARSI, Indonesia
razari universitas yarsi, Indonesia
harliansyah harliansyah universitas yarsi, Indonesia
syukrini bahri

DOI:

https://doi.org/10.36990/hijp.v16i1.1123

Keywords:

Stunting, metilasi DNA, gen IGF2

Abstract

Ringkasan: Latar Belakang: Stunting dengan prevalensi 24% pada anak Indonesia berkaitan dengan kekurangan donor metil yang mengatur ekspresi gen pertumbuhan melalui metilasi DNA gen IGF2. Tujuan: Mengevaluasi perbedaan metilasi DNA gen IGF2 pada anak stunting sebagai penanda biologis. Metode: Studi case-control retrospektif melibatkan 46 anak usia 3-5 tahun (23 stunting, 23 normal) di Pandeglang, Banten. DNA darah tepi dianalisis menggunakan Methylation Specific PCR dengan kit CpG WIZ H19-IGF2. Analisis statistik menggunakan uji Kruskal-Wallis. Hasil: Metilasi DNA gen IGF2 lebih tinggi pada anak stunting (median 22% vs 17,83%, p>0,05) namun tidak bermakna statistik. Metilasi bermakna lebih tinggi pada anak laki-laki (median 28,68% vs 14,80%, p=0,04). Simpulan: Metilasi DNA gen IGF2 berpotensi menjadi penanda biologis stunting, terutama pada anak laki-laki. Saran: Diperlukan penelitian lanjutan dengan sampel lebih besar untuk mengeksplorasi mekanisme molekuler stunting.

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